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Unlike growth hormone–related peptides that act via the pituitary, TB‑500 works directly at the site of injury to stimulate healing across a wide range of tissues—including tendons, muscles, fascia, ligaments, and even cardiac tissue

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To examine the nitric oxide-dependency in this system, the rings were pretreated with 0.03 mM L-NAME, an eNOS inhibitor or 10 μM hemoglobin, a nitric oxide scavenger