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Coli containing expressed target fusion protein PBS/EDTA: 1X PBS, 5 mM EDTA, pH 7.4 Lysis buffer: 50 mM Tris, 50 mM NaCl, 5 mM EDTA, 1 μg/ml leupeptin, 1 μg/ml pepstatin, 0.15 mM PMSF, 1 mM DFP, 1 mM 2-ME, pH 8.0

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22 - 25 Background of the Robert’s concept (stomach cytoprotection and organoprotection) versus the Selye’s concept (stress coping response) Robert’s stomach cytoprotection 14 - 19 and organoprotection 20 , 21 and the Selye’s stress coping response 22 - 25 both intend to reestablish homeostasis